In vitro sporulation, oocysticidal sporulation inhibition of Eimeria papillate and cytotoxic efficacy of methanolic extract of Thymus daenensis leaves Maodaa Saleh N.1,*, Al-Shaebi Esam M.1, Hailan Waleed Ali Qaid1, Abdel-Gaber Rewaida1, Alatawi Afaf1, Alawwad Sarah A.2, Al-Quraishy Saleh1 1Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia 2Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia *Corresponding Author: Saleh N. Maodaa, Department of Zoology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia, Email: maodaa_28@yahoo.com
Online Published on 8 February, 2024. Abstract Background Thymus daenensis is a phenol-rich species of the Thymus genus that possesses many biological and pharmaceutical activities. Methods This study used five groups of TDLE extract (50, 100, 200 and mg/mL). Distilled water and mebendazole (10 mg/ml) were used as negative and positive controls. Five worms of similar size were placed in each Petri plate, together with the extract test solution (20 mL). The present work aims to study the phytochemical profiling, evaluation of anticancer properties and in vitro oocysticidal activity of methanolic T. daenensis leaves extracts (TDLE). Result Infrared spectroscopy (FT-IR) of TDLE proved eleven different active classes of chemical compounds. The extract is characterized by higher phenolic contents (250.5±2.7 mg/g of gallic acid) and flavonoids (25±0.3 mg/g of quercetin). Also, TDLE showed moderate cytotoxicity against breast cancer cell lines (MCF-7) and lung cancer cell lines (A549) using MTT assay with LC50 attributed to 388.66±3.5 μg/ml and 354.33±2.5 μg/ml, respectively. The in-vitro study revealed that TDLE concentrations of 300 mg/ml caused significant suppression of Eimeria. papillata oocysts sporulation and increased percent of sporulation inhibition compared to the other commercial products which showed different levels of sporulation. Our findings indicate that TDLE has anticoccidial activity, which encourages the conduct of numerous In vivo investigations to find an effective treatment. Top Keywords Anticoccidial, Cytotoxicity, E. papillata, Thymus daenensis. Top |