In vivo and in vitro study to evaluate the anti-osteoporotic activity of Punica granatum seed, Bambusa arundinaceae leaves and Trichosanthes diocio fruit ethanolic extract Awasthi Akanksha1,*, Juyal Divya2, Singh Mamta F.3, Sharma Saurabh1 1Vivek College of Technical Education, Bijnor-246 701, Uttar Pradesh, India 2Roorkee College of Engineering, Bajuhari, Roorkee-247 667, Uttarakhand, India 3Sardar Bhagwan Singh University, Balawala, Dehradun-248 161, Uttarakhand, India *Corresponding Author: Akanksha Awasthi, Vivek College of Technical Education, Bijnor-246 701, Uttar Pradesh, India, Email: akankashaawasthi333@gmail.com
Online Published on 8 February, 2024. Abstract Background In the present study, it was aimed at evaluating the efficacy of ethanolic extract of Punica granatum seed, Bambusa arundinaceae leaves and Trichosanthes diocio fruit on their anti-osteoporotic activity in ovariectomized (OVX) rats and mcf-7 cell line to identify their osteoprotective role. Methods Thirty six female albino wistar OVX rats were treated with the ethanolic extract of Punica granatum seed, Bambusa arundinaceae leaves and Trichosanthes diocio fruit for 45 days. The ethanolic extracts were given in different concentrations for 24 h and a cytotoxicity study was carried out by trypan blue dye exclusion assay. Result The ethanolic extract of Punica granatum seed, Bambusa arundinaceae leaves and Trichosanthes diocio fruit treated notably averted an OVX-induced increase in body weight. Moreover, exposure to ethanolic extract of Bambusa arundinacea significantly decreased lipid peroxidation and protein carbonyl formation. Treated mcf-7 cell line viability was also increased. All these finding suggest that the ethanolic extract of Punica granatum seed, Bambusa arundinaceae leaves and Trichosanthes diocio fruit have an important role in reducing osteoporosis. Top Keywords Ethanolic extract, In vitro study, Mcf-7 cell line, Ovariectomized rats. Top |