Prediction of miRNA targets affected proteins and their homologs in Mouse gammaherpesvirus68 Gautam Budhayash1,*, Katara Pramod2, Choudhary Anshu1, Wadhwa Gulshan3, Singh Satendra1 1Department of Computational Biology & Bioinformatics, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences-Deemed to be University, Allahabad-211007, India 2Department of Bioscience and Biotechnology, Banasthali University, P.O. Banasthali Vidyapith, India-304022 3Apex Bioinformatics Centre, Department of Biotechnology, Ministry of Science and Technology, CGO Complex, Lodhi Road, New Delhi – 110 003 *Corresponding Author: Budhayash Gautam, Email: budhayash.gautam@shiats.edu.in
Online published on 25 April, 2013. Abstract microRNAs are small noncoding, single-stranded RNA gene product about 20-24nt long that are processed by Dicer from precursor with a characteristic hairpin secondary structure. Precise regulation of miRNAs activity during various stages of growth and in specific cell types is of central importance for normal development because miRNAs affect morphology of plants and animals by regulation of the gene expression at the post-transcriptional level which is involved in critical developmental events. Thus, in the present study the focus is on the animal miRNAs and prediction of the miRNA target, affected proteins by miRNA and miRNA homolog of Mousegammaherpes virus68. Present analyses are based on sequence complimentaries between miRNA and mRNAs. As a result, we predicted 98 targets for 49 mature miRNA sequences and among these 58 mature miRNA sequences were already published in database. The study of affected proteins revealed that very less number of miRNAs, protein products are known and they mostly involved in diverse processes like elements of signal recognition. Homology analyses for miRNAs suggested that 17 miRNAs of Mousegammaherpes virus68 show 379 miRNA homologs for different animal species. Top Keywords miRNA targets, in silico identification, Mousegammaherpes virus68, homologs, EST. Top |