Genotoxic Effect of Synthetic Pyrethroids on Fresh Water Fish Channa Punctatus Dr. Rana Rupali Pest and Parasite Research Laboratory, Deptt. Of Zoology, Bareilly College, Bareilly, India Online published on 21 November, 2017. Abstract In this study, two synthetic pyrethroid insecticides cypermethrin and permethrin were tested for its genotoxicity in a fresh water fish Channa punctatus. The number of chromosomes in this fish was 32 in normal diploid cells. Out of these 32 chromosomes, the number of metacentric, submetacentric, acrocentric and telocentric chromosomes was 14, 8, 4 and 6, respectively In chromosomal analysis when the fishes were exposed to three defferent concentrations (0.0020 ppm, 0.0025ppm and 0.0035ppm) of cypermethrin and (1.0, 1.2 and 2.4 ppm) of permethrin and sacrificed at three sampling times (96, 120 and 144 hours) the number of Structural Chromosomal Aberrations (SCA) were increased in kidney cells. Results obtained demonstrated the genotoxic effects of the pyrethroid in the experimental model. It was observed that after exposure to cypermethrin for 144 hrs the percentage of spreads with chromosomal abnormalities was 78.2%, 82.2% and 79.1% and when exposed to permethrin for 144 hrs the percentage was 82.5%, 56.5% and 88.5% in kidney cells of Channa punctatus. These changes were dependent upon period of exposure and concentration of cypermethrin and permethrin. The cypermethrin and permethrin induced structural chromosomal aberrations, which induced chromatid breaks, fragments, gap, chromatid separation, acentric fragment, deletion and ring type chromosomes in experimental fish. Top Keywords Genotoxicity, Cypermethrin, Permethrin, Structural chromosomal aberration test, Channa punctatus. Top |