Effect of growth conditions on extracellular α-amylase production by Bacillus thuringiensis Tembhurkar V.R.1,*, Bannatwala M.K.2, Udare S.S.3, Kalyankar M.G.4 1Department of Microbiology, ASC College, Jalna, India 2Department of Medical Microbiology, Greater Kailash Hospital, Indore, India 3Department of Biotechnology, MGM College, Aurangabad, India 4Deptepartment of Biotechnology, University of Houston, Clear Lake, USA *Email: vrtembhurkar@gmail.com
Online published on 19 March, 2013. Abstract Influence of few growth conditions and media components on alpha amylase production by Bacillus thuringiensis in lab scale batch process were determined. B. thuringiensis produced maximum amylase after 72hrs of incubation. The optimum temperature and pH for amylase production were found to be 31°C and 7, respectively. The optimized media composed of [g/l] 10.0g Rice flour; 5.0 Peptone; CaSO4 4.0; MgSO4 2.0; NaCl 1.0; FeSO4 0.5 and pH 7. The alpha amylase produced was working optimally at 60°C, pH 5, [S] 20 mg/mL, [E] 2.0 mL. Many of these parameters are in compliance with the insecticidal crystal protein production by B. thuringiensis. Therefore, the process can be used for simultaneous production of alpha amylase and bioinsecticide in a single fermentation cycle. Top Keywords Bacillus thuringiensis, Alpha amylase, Insecticide Crystal proteins, growth. Top |