Cytoplasmic localization of p38 MAPK and MDM2 expression which mediate p53 expression in canine skin tumours Kumar Pawan2,*, War Z.A.2, Dar J.A.2, Mishra Sonali2, Bindhuja B.V.1,2, Sharma A.K.2, Singh Rajendra2, Singh Vidya2 2Division of Pathology, ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India 1Division of Surgery, ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India *Corresponding author: chauhan2k1@gmail.com
Abstract P53, MDM2 and p38 MAPK are involved in regulation of cell growth and their altered expression may lead to the malignant transformation of the cell. In canine tumours, over expression of p53 was related to the malignancy and poor prognosis. The aim of the present investigation was to evaluate the relationship of p53 with nuclear and cytoplasmic localization of MDM2 and p38 MAPK in canine skin tumour. Seventeen tumour cases were included in the present study; among them 8 were diagnosed as benign while 9 were malignant. P53 expression was detected in nucleus and it was higher in malignant tumours than benign. MDM2 and p38 MAPK expression was found in both nucleus and cytoplasm. It was observed that cytoplasmic p38 expression was positively related with the p53 expression. MDM2 positive cases where it was localized in cytoplasm showed comparatively lower expression of the p53. Besides, it was observed that nuclear expression of both MDM2 and p38 MAPK did not show a regular pattern of association between them and with p53. In conclusion, we found that cytoplasmic localization of the p38 MAPK may have a role in increased expression of the p53 while MDM2 cytoplasmic localization negatively related with p53 expression in canine skin tumours. Top Keywords Canine, MDM2, p38 MAPK, p53, Skin tumour. Top |