Antimicrobial resistant Escherichia coli and associated colibacillosis in poultry population of Mizoram Jamoh K.1, Rajkhowa T.K.1,*, Singh Y.D.1, Ravindran R.1, Arya R.S.1 1Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram-796014, India *Corresponding author: e-mail: tridibraj09@gmail.com
Abstract Incidence and pathology of colibacillosis was studied in poultry population, covering 18 commercial poultry farms located in different districts of Mizoram, India. A total of 504 E. coli isolates from heart blood of 203 birds died due to colibacillosis were characterized by bacteriological, biochemical and molecular techniques. Antibiogram study was conducted to establish the antimicrobial resistance of all the isolates. Further, Extended-spectrum beta-lactamases (ESBLs) production ability of the isolates was determined by double discs synergy test (DDST) method. The colisepticaemic form of the disease was observed as the most common form of colibacillosis followed by salphingitis, oophoritis, egg peritonitis and mushy chick disease in poultry population of Mizoram. The most consistent pathological lesions observed were fibrinous pericarditis and perihepatitis. E. coli isolated from different forms of colibacillosis revealed antibiotic resistance against up to 13 antibiotics. Antibiotics with highest level of resistance was recorded against Amoxicillin (87.23%) followed by nalidixic acid (80.75%), tetracycline (75.91%) and ampicillin (41.43%), with minimum resistance against imipenem (1.22%). A total of 40.47% (204/504) isolates were confirmed as ESBL producers by DDST screening method. Top Keywords Antimicrobial resistance, Colibacillosis, ESBLs, Escherichia coli, Mizoram. Top |