Year : 2018, Volume : 42, Issue : 3
First page : ( 202) Last page : ( 204)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2018 September 1.
Article DOI : 10.5958/0973-970X.2018.00048.2

Sero-diagnosis of paratuberculosis in Gaddi sheep of Himachal Pradesh

Maity Madhulina¹, Thakur Monika¹, Gupta V.K.^1,*

¹Department of Veterinary Pathology, Dr G.C. Negi College of Veterinary & Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur-176062, Himachal Pradesh, India

*Corresponding author: e-mail: gupta.vipankumar@gmail.com

Received:  29  May,  2018; Accepted:  22  August,  2018.

Abstract

The present study was designed to evaluate the sero-prevalence of paratuberculosis inmigratory Gaddi sheep of Himachal Pradesh using indigenous ELISA kit. Thirty nine serum samples obtained from slaughtered sheep from the local slaughter places at Palampur during September 2013 to March 2014 were tested against Mycobacterium avium subsp. Paratuberculosis (MAP). Out of 39 sera screened, 20 (51.28%) were found to be positive. Moreover, ELISA detected the highest percent (63%) positive cases from the age group above 2 years in comparison to sero-positive younger animals. Whereas, agar gel immunodiffusion (AGID) assay was not able to detect any positive case. Also, acid-fast staining (Ziehl-Neelsen) in cytosmears could detect only 7 (18%) and none in tissue sections. The ELISA positive cases revealed histological lesions ranging from Grade-II to IV. It was concluded that paratuberculosis was prevalent in Gaddi sheep population of Himachal and the indigenous ELISA was specific and sensitive as compared to AGID or acid-fast staining in detecting positive cases.

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Keywords

ELISA, Gaddi sheep, MAP, Paratuberculosis, Sero-diagnosis.

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