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Sero-diagnosis of paratuberculosis in Gaddi sheep of Himachal Pradesh Maity Madhulina1, Thakur Monika1, Gupta V.K.1,* 1Department of Veterinary Pathology, Dr G.C. Negi College of Veterinary & Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur-176062, Himachal Pradesh, India *Corresponding author: e-mail: gupta.vipankumar@gmail.com
Abstract The present study was designed to evaluate the sero-prevalence of paratuberculosis inmigratory Gaddi sheep of Himachal Pradesh using indigenous ELISA kit. Thirty nine serum samples obtained from slaughtered sheep from the local slaughter places at Palampur during September 2013 to March 2014 were tested against Mycobacterium avium subsp. Paratuberculosis (MAP). Out of 39 sera screened, 20 (51.28%) were found to be positive. Moreover, ELISA detected the highest percent (63%) positive cases from the age group above 2 years in comparison to sero-positive younger animals. Whereas, agar gel immunodiffusion (AGID) assay was not able to detect any positive case. Also, acid-fast staining (Ziehl-Neelsen) in cytosmears could detect only 7 (18%) and none in tissue sections. The ELISA positive cases revealed histological lesions ranging from Grade-II to IV. It was concluded that paratuberculosis was prevalent in Gaddi sheep population of Himachal and the indigenous ELISA was specific and sensitive as compared to AGID or acid-fast staining in detecting positive cases. Top Keywords ELISA, Gaddi sheep, MAP, Paratuberculosis, Sero-diagnosis. Top | ACKNOWLEDGEMENTS The authors are thankful to the Head of the Department of Veterinary Pathology and Dean, College of Veterinary Animal Sciences for providing the facilities and funds to undertake the study. It would not have been possible to undertake the study without the help and support of the slaughter men in collecting the samples for the present study. |
Top Tables | Table 1.: Criteria set for ELISA score according to S/P ratio
| | S/P ratio | ELISA Score | | >1 | Strongly positive | | 0.4–0.99 | Positive | | 0.25–0.39 | Low positive | | 0.1–0.24 | Suspected | | 0–0.09 | Negative |
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| S/P ratio of 0.4 or above was considered as positive and S/P ratio of 0.39 and below was considered as negative. | | | Table 2.: Comparison of ELISA score with histopatholog- ical lesions grade, Agar gel immunodiffusion (AGID) and Ziehl-Neelsen (ZN) staining.
| | Animal No. | Histo pathological12 lesions grade | AGID | ELISA score (S/P ratio) | ZN staining | | Smear cytology | Tissue sections | | 1.Sh-R-001/13 | III | SP (1.434) | N | P | N | | 2.Sh-R-006/13 | IV | P (0.621) | N | N | N | | 3.Sh-R-007/13 | III | SP (1.179) | N | P | N | | 4.Sh-R-009/13 | II | P (0.484) | N | N | N | | 5.Sh-R-015/13 | III | P (0.563) | N | P | N | | 6.Sh-R-016/13 | III | P (0.526) | N | N | N | | 7.Sh-R-019/13 | II | P (0.487) | N | N | N | | 8.Sh-R-020/13 | II | P (0.510) | N | N | N | | 9.Sh-R-022/13 | IV | P (0.640) | N | N | N | | 10.Sh-R-023/13 | II | P (0.486) | N | P | N | | 11.Sh-R-025/13 | II | P (0.476) | N | N | N | | 12.Sh-R-027/13 | III | P (0.720) | N | P | N | | 13. Sh-R-030/13 | II | P (0.617) | N | P | N | | 14.Sh-R-031/13 | III | P (0.703) | N | P | N | | 15.Sh-R-032/13 | II | P (0.443) | N | N | N | | 16.Sh-R-033/13 | II | P (0.551) | N | N | N | | 17.Sh-R-035/13 | II | P (0.557) | N | N | N | | 18. Sh-R-036/13 | II | P (0.786) | N | N | N | | 19. Sh-R-038/13 | II | P (0.673) | N | N | N | | 20.Sh-R-039/13 | III | P (0.758) | N | N | N |
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