Year : 2018, Volume : 42, Issue : 3
First page : ( 213) Last page : ( 216)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2018 September 1.
Article DOI : 10.5958/0973-970X.2018.00052.4

Nodular hepatocellular carcinoma in a Labrador dog with metastases

Sankaranarayanan Hemalatha², Hemalatha S.^2,*, Sridhar R.^2,1

²Department of Veterinary Pathology, Madras Veterinary College, Chennai-7;

¹Department of Veterinary Pathology, VCRI, Tirunelveli-627358, Tamil Nadu, India

*Corresponding author: e-mail: hemacul@yahoo.com

Received:  9  May,  2018; Accepted:  15  August,  2018.

Abstract

A 10 year old male Labrador retriever was presented for necropsy with the clinical history of vomition and pain on palpation of abdomen. Ultrasound examination revealed enlarged and abnormal liver borders. Haemato-biochemical studies revealed anaemia, leu-kocytosis and elevated liver specific enzymes. Necropsy examination of the carcass revealed mottled liver with multiple variably sized round, discrete to coalescing grey white to brown soft friable nodules on all the lobes. A few nodules were cystic with accumulation of viscid fluid. Similarly, multifocal variable sized dark red nodules were also found randomly scattered on both the lungs. Pancreas and kidneys also revealed metastasis. Histologically, the liver nodules appeared as nodular hepatocellular carcinoma with adenoid pattern. Metastatic foci were observed in the lungs, pancreas and kidneys. Immunohistochemical detection of tumour markers such as alpha fetoprotein, proliferating cell nuclear antigen, pancytokeratin, vimentin, chorioembryonic antigen were carried out for differential diagnosis.

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Keywords

Dog, Hepatocellular carcinoma, Immunohistochemistry, Metastases, Pathology.

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Figures

Fig. 1.: Liver showing mottling with variable sized nodules TopBack

Fig. 2.: Cut section of liver showing grey white & cystic nodules TopBack

Fig. 3.: Lungs showing multifocal red raised areas TopBack

Fig. 4.: Cut section of pancreas showing multifocal grey white nodules TopBack

Fig. 5.: Liver showing adenoid pattern of tumour cells H&E ×400 TopBack

Fig. 6.: Liver showing pleomorphic tumour cells with anisokaryosis, multiple nucleoli H&E ×400. TopBack

Fig. 7.: Lung showing metastatic foci resembling primary tumour H&E ×100 TopBack

Fig. 8.: Pancreas showing emboli in interacinar vessel H&E ×400 TopBack

Fig. 9.: Alpha fetoprotein- Intense positive reaction in hepatocytes IPTx400 TopBack

Fig. 10.: PCNA positive nuclear staining in hepatocytes IPTx400 TopBack

Fig. 11.: Alpha fetoprotein- Intense positive reaction in metastatic focus in lungs IPTx400 TopBack

Fig. 12.: Pancytokeratin-Positive cytoplasmic staining in hepatocytes IPTx400. TopBack