Clinico-pathological effects of copper nanoparticles in Wistar rats Mewari Neeraj Singh*, Chauhan R.S., Shodhan K.V., Kandpal Diksha, Neha Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India. Address for Correspondence Dr Neeraj Singh Mewari, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India, E-mail: neerajmewari31092@gmail.com
Abstract Nanotechnology has revolutionized the commercial application of nanomaterials in the fields of medicine, engineering, information, environmental technology, pigments, food, electronics, home appliances, biological applications, organic and pharmaceutical packages, etc. The synthesis of copper nanoparticles is cost efficient and the synthesized copper nanoparticles exhibit antimicrobial activity against many pathogenic microorganisms. However, it is necessary to study clinico pathological effects of the oral exposure of nano-copper in Wistar rats. For this study, a total of 35 rats of both the sexes were randomly divided into two groups viz. group 1 and group 2. Group 1 was kept as control and consisting of 20 rats. Group 2 was nano-copper treated and comprising of 15 rats. The rats in group 1 were provided with standard diet and water, while the rats in group 2 along with standard recommended feed and RO water, were additionally given copper nanoparticles mixed in distilled water orally at No Observed Adverse Effect Level (NOAEL) dose rate of 100 mg/kg body weight/day from 0 day of experiment till 90th day post treatment (DPT). Clinico pathological studies were carried out at 0, 30th, 60th and 90th DPT and haematological and biochemical parameters were assessed. Haematological parameters showed decrease in the mean hemoglobin, mean packed cell volume, mean total leucocyte count, mean absolute lymphocyte count and mean lymphocyte count in nano-copper treated group as compared to control group. There was an increase observed in absolute neutrophil count and mean neutrophil count. Biochemical parameters like total serum protein, serum albumin, serum globulin and serum gamma globulin showed decrease in values in nano-copper treated group as compared to control group. The present study can be concluded that the nano-copper in NOAEL (No Observable Adverse Effect Level) dose is exerting its deleterious effects on vital organs of the body that may lead to immunopathology and may adversely affect the reproductive health of animals. Top Keywords Clinico pathological studies, DPT, Immunopathology, Nano-copper, NOAEL. Top |