The present investigation was carried out to study pathomorphological studies of diseases in wild felids with special reference to canine distemper. Of 116 clinical samples, CDV was found in two cases each of lions and leopards by RT-PCR. Twenty two of 129 (17.05%) cases of wild felids were found positive for CDV infection by histopathology and immunohistochemistry. The major microscopic lesions were observed in spleen, lung, kidney and brain. Most specific and consistent histopathological lesions were observed in spleen which included moderate to severe degree of lymphoid depletion and necrosis of lymphoid cells in follicles, formation of syncytia and presence of intranuclear and intracytoplasmic eosinophilic inclusion bodies. Out of 73 tissue samples stored at −20°C, 23 (31.51%) cases were found positive for CDV genome by RT-PCR. Phylogenetic analysis by Nabor-joining method, the strain of CDV formed separate clad with Onderstpoort vaccine strain and strain from spotted hyena in South Africa. Indian isolates of lion formed separate clad with that of Indian isolates from dog and palm civet cat. Two brain samples one each of jungle cat and leopard were found positive for rabies which were confirmed by dFAT and RT-PCR. Presence of intracytoplasmic inclusion “Negri bodies” were detected by Seller's staining. Sequence analysis of N gene revealed that Indian isolates belonged to Arctic like 1a clad. Seroprevalance of leptospira by MAT was found to be 60.00% (27/45) in tigers, 64.19% (52/81) in lions and 65.51% (19/29) in leopards. The more frequently occurring serovars were Icterohaemorrhagiae and Grippotyphosa. Of 72 faecal samples parasitic infestation was found in 22 (30.55%) wild felids and exhibited presence of eggs of Toxocari cati, Spirometra sp., Taenia sp., Ancylostoma sp. and Physaloptera sp. At necropsy Toxocara cati, Taenia taeniaeformi sp., Ancylostoma sp. and Dirofilaria immitis were recovered. The parasitic stages migrating through the tissues were also observed in 8 formalin fixed tissues. A total of 15/129 tumour cases were diagnosed, out of these, highest occurrence of adenocarcinoma (4/15, 26.66%) followed by squamous cell carcinoma (3/15, 20.00%), fibroma (2/15, 13.33%) and one each of fibrosarcoma, fibrous histiocytoma, hemangiosarcoma, lymphoblastic leukaemia, leiomyoma and cholangiocellular carcinoma was found. The nonspecific disease/condition were observed in kidneys (23/129, 17.82%), followed by in lungs (22/129, 17.05%) and liver (16/129, 12.40%). Metastatic calcification was observed in two tigers and in one lion cub. Traumatic injuries were recorded in 42.10% (8/19) cases. It was concluded that CDV is circulating in wild felids and causing diseases in them. Other important condition diagnosed were rabies, leptospira and tumours.