Carrier status of mycoplasmoses in the apparently healthy pigs of India Thakor Jigarji Chaturji1, Patel Sagar1, Murali Dinesh1, Sahoo Monalisa1,5,**, Kumar Ajay4, Saminathan M.2, Singh Karam Pal2*, Singh Rajendra1, Kumar Sai1, Chauhan Anuj3 1Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India 2ICAR-CADRAD, Indian Veterinary Research Institute (IVRI), Izatnagar, India 3Division of Animal Genetics and Breeding, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India 4Division of Biochemistry, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, India 5ICAR-DFMD-International Centre for Foot and Mouth Disease, Arugul, Jatni, Bhubaneswar, Odisha *Address for Correspondence: Karam Pal Singh, ICAR-CADRAD, Indian Veterinary Research Institute (IVRI), Izatnagar, India, E-mail: karam.singh@rediffmail.com
**Monalisa Sahoo, ICAR-DFMD-ICFMD, Arugul, Jatni, Bhubaneswar, Odisha-752 050, India, E-mail: vety.lisa@gmail.com
Online Published on 20 June, 2023. Abstract Mycoplasma is an emerging bacterial pathogen confronting global swine industry. The carrier pigs play major role in the transmission of disease to the naïve population. The carrier status of Mycoplasmoses in clinically healthy (live and slaughtered) pigs of India is not known and needs to be investigated for its effective control. A total of 494 morbid tissues (palatine tonsil and lungs) and 508 nasal swabs collected from the apparently healthy slaughtered and live pigs respectively, were screened by PCR for prevalence of Mycoplasma and its species. The prevalence of Mycoplasma genus was 43.92% and 53.74% in slaughtered and live pigs respectively. The palatine tonsils showed higher carrier rate (26.72%) as compared to lungs. Out of various spp, M. hyorhinis is prevalent as compared to others. The microscopic lesions of lymphoid aggregates in perivascular and peribronchiolar area of lungs, depleted lymphoid follicles in palatine tonsil with immunoreactivity of M. hyorhinis antigen in lungs and palatine tonsil confirm its presence in carrier pigs. The phylogenetic analysis showed close homology of our isolates with Israel isolate. The effective control and preventive measures should be adopted to contain the spread of M. hyorhinis. Top Keywords Carrier pigs, Immunohistochemistry, Mycoplasma, Mycoplasma hyorhinis, Pathology, PCR. Top |