Effect of 5-amino salicylic acid on oxazolone induced ulcerative colitis in adult zebrafish model Srinivasan M.R.1, Janani M.2, Ramasamy T.3, Madheswaran R.4,* 1Laboratory Animal Medicine, Centre for Animal Health Studies, TANUVAS, Chennai-600 051, Tamil Nadu 2Department of Biotechnology, Rajalakshmi Engineering College, Anna University, Thandalam, Chennai-602 105 3Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, TANUVAS, Chennai-600 007 4Department of Veterinary Pathology, Veterinary College and Research Institute, TANUVAS, Udumalpet-642 205, India *Address for Correspondence R. Madheswaran, Department of Veterinary Pathology, Veterinary College and Research Institute, TANUVAS, Udumalpet-642 205, India, E-mail: rmadheswaran2003@gmail.com
Online Published on 2 January, 2024. Abstract Ulcerative colitis was induced in adult zebrafish using oxazolone and treated with non-steroidal anti-inflammatory drug, 5-amino salicylic acid (5-ASA). The experiment was conducted into two phases as tolerability and effcacy studies using one hundred adult zebrafish. Tolerability study was conducted to ascertain the maximum tolerable dose of 5-ASA using 30 zebrafish. The fishes were randomly distributed to six groups of five fish in each group and duration of the study was eight days. Effcacy study was conducted to find out the anti-inflammatory effects of 5-ASA in oxazolone induced colitis using 70 zebrafish. The fishes were randomly allocated to seven groups of ten fish in each group and duration of the study was six days. Colitis was induced in zebrafish by intrarectal administration of oxazolone on the first day. The colitis induced fishes were treated with 5-ASA through oral route for four consecutive days from 2-5. Fishes were sacrificed on day six and intestine of five fishes from each group was collected for histopathology. The inflammatory changes in the colonic mucosa like thickening of bowel wall, disruption of mucosal architecture, presence of goblet cells and infiltration of eosinophils were included for histological scoring. The changes were reversed after treatment with 5-ASA at 2 µg/fish and the reversal was not dose dependent in higher doses. Further studies are required to establish the dose response curve for the dose less than 2 µg/fish. Hence, this model can be explored as a replacement for ulcerative colitis mouse model to study the effcacy of the drugs. Top Keywords 5-ASA, Histopathology, Oxazolone, Ulcerative colitis, Zebrafish. Top |