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Allelic frequency of κ-casein and β-lactoglobulin in Jersey cattle Shetty Savita**, Patel R.K.1*, Soni K.J.1, Singh K.M.1, Chauhan J.B.1 1R&D-Biotechnology, National Dairy Development Board Anand-388 001 (Guajart) *Corresponding author: R K Patel, Scientist – III (R&D-Biotechnology), Indian Immunological Ltd., Hyderabad. E-mail: rkpatel46@yahoo.com
**Part of the M.Sc (Biotechnology)
Summary A Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed on DNA samples extracted from blood samples of Jersey cattle to detect genotype frequency of the bovine kappa casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa casein and 247 bp fragment for beta-lactoglobulin was amplified and digested with Hinf I and Hae III restriction enzymes respectively. Two types of alleles A and B and three types of genotypes AA, BB and AB for kappa casein and beta-lactoglobulin were observed in the study. The results indicated predominance of B-allele for both traits that are useful markers for milk production traits on which bull can be evaluated and selected for future breeding programmes. Top Key Words β-Lactoglobulin, Jersey, κ-Casein, PCR-RFLP, Genotype. Top | | | |
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