Therapeutic effects of hesperidin on N-methyl-nnitrosourea induced mammary tumors in rats Urkude P. Atul1,3,*, Vihol D. Priti1, Patel Jignesh M.1, Patel Jatin H.2, Varia Raseshkumar D.2, Saurathiya Vishal L.1, Dalvi Milind U.1 1Department of Veterinary Pathology, Navsari Agricultural University, Navsari, Gujarat, India 2Department of Veterinary Pharmacology and Toxicology, Navsari Agricultural University, Navsari, Gujarat, India 3Department of Veterinary Pathology, Navsari Agricultural University, Navsari, Gujarat, India *Correspondence author's e-mail: at.atul.94@gmail.com
Online published on 28 November, 2023. Abstract Millions of new cases of fatal mammary cancer are diagnosed every year in animals. The present study evaluate therapeutic effects of hesperidin on N-methly-N-nitrosourea (MNU) induced mammary tumors in Sprague- Dawley (SD) rats with reference to hematology, biochemistry, histopathology and immunohistochemical expressions of PCNA, Ki-67 and VEGF markers. Total 14 out of 18 MNU exposed rats developed mammary tumors which were then classified histopathologically. At the end of the study, TEC, Hb and PCV levels were restored in hesperidin treatment groups. Moreover, hepatoprotective activity of HES against MNU induced toxicity was revealed with quite normal values of ALT, TP and ALB in hesperidin treatment groups. Histopathologically invasive papillary carcinoma followed by invasive cribriform carcinoma was found to be most common mammary tumors in rats. Dose dependent anti-hyperproliferative activity of HES in MNU induced mammary tumors was noted by assessing the immunohistochemical expression of PCNA and Ki-67 markers in mammary tumors of rats. While, antiangiogenic activity of HES in neoplastic cells was seen only at higher dose rate. Top Keywords MNU, Hesperidin, Mammary tumors, Hisopathology, IHC, Hematology, Biochemistry. Top |