Effect of polyherbal formulation on oxidative stress and genotoxicity in lactating holstein friesian crossbred cattle Jain Surbhi1, Lonare M. K.1, Singla Saloni1, Sharma Manjinder2, Lonare Milindmitra K1,*, Kaur Simarjeet3 1Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India 2Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India 3Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India *Corresponding author's e-mail: milindmitra@gmail.com
Online published on 28 November, 2023. Abstract The present study was undertaken to determine the effect of a polyherbal formulation (galactagogue) supplementation on oxidative stress and genotoxicity in lactating Holstein Friesian (HF) crossbred cattle. Animals were randomly and equally divided into three groups, with ten animals in each group. Animals were fed with and without polyherbal formation for daily along with regular lactating diet for 45 days. Group I (Control animals), received a regular lactating diet. Group II received synthetic galactagogue (domperidone @ 10mg/100kg/day, orally). Group III, received a polyherbal formulation (20g/100kg body weight twice a day). Anti-oxidative stress parameters were evaluated on days 0, 30, and 60. Genotoxicity parameters were evaluated on day 60. A significant decrease (p<0.05) in LPO, while, a significant increase (p<0.05) in SOD and GSH was noticed in group III. There was a significant (p<0.05) rise in CAT in group II and a significant (p<0.05) decline in the GPx levels in all groups. Comet assay data revealed no persistent or long-term effects on DNA damage with polyherbal formation.Non-significant changes (p>0.05) in tail DNA percent,tail length, tail moment, and olive moment were noticed when compared with the control group I, while synthetic galactogogue showed marked significant (p<0.05) DNA damage compared to the control. The study concluded that polyherbal galactagogue supplement does not have an adverse effects or delayed genotoxic effect in a lactating cattle. Moreover, it has a generalized positive impact on the body's performance by mitigating oxidative stress arises during production with no genetoxic effect on long term use. Top Keywords Polyherbal, Galactagogue, Domperidone, Antioxidant, Genotoxicity. Top |