The Separation, Isolation, Purification and Characterisation of IgG from Non-Epileptic and Epileptic Patients Singh Vijender1,*, Khan Moinuddin2, Chauhan Manju3 1Ph.D Student, Department of Biotechnology, Singhania University, Jhunjhunu, Rajasthan, India 2Professor and VC, Department of Administration, Singhania University, Jhunjhunu, Rajasthan, India 3Professor and Head of Department of Bioscience, D.A.V. (PG) College, Muzaffar Nagar, Uttar Pradesh, India *Email id: vijendersingh2005@yahoo.co.in
Abstract Research work on the separation, isolation, purification and characterisation of IgG from non-epileptic and epileptic patients was carried out to examine the unexplored and overcome neurological disorders (epilepsy) with the help of various sophisticated techniques. The results were summarised as follows: (1) The IgG conc. in blood serum of epileptic patients increased as compared to the non-epileptic persons. (2) The IgG conc. in blood serum of non-epileptic and epileptic patients, studied by UV spectroscopy, showed similar results. The increase in IgG conc. in blood serum of epileptic patients is due to increased level of neuron-specific antibodies in the sera. (3) The molecular characterisation of IgG was studied by disc gel electrophoresis. A single protein band was observed in a normal person's pattern; whereas, the protein profile of IgG in abnormal serum samples (E1, E2 and E3) shows one or two protein bands of both IgG and IgA. Top Keywords Separation, Isolation, Purification and Characterisation of Protein IgG, Blood serum, Non-Epileptic and Epileptic Patients. Top |