Echinacea purpurea- a host of 16SrXII-A phytoplasma group in Serbia Pavlovic Snezana1, Starovic Mira2, Stojanovic Sasa2, Popovic Tatjana2, Aleksic Goran2, Drazic Slobodan1, Josic Dragana3,* 1Institute for Medicinal Plant Research “Dr. J. Pancic”, Belgrade, Serbia 2Institute for Plant Protection and Environment, T. Drajzera 9, Belgrade, Serbia 3Institute of Soil Science, Genetic laboratory, T. Drajzera 7, Belgrade, Serbia Corresponding author e-mail: *josicdragana@yahoo.com
Abstract During a survey carried out from 2005 to 2010, reddening, stunting and proliferation of axillary shoots or witches’ broom symptoms were observed on Echinacea purpurea in Pancevo and Indjija, Serbia. The association of phytoplasma with these symptoms has been detected by electron microscopic observations and PCR followed by nested PCR using phytoplasma 16S rRNA gene specific primers. Further, 16SrXII-A group (stolbur) phytoplasmas were identified by RFLP analysis using TruI, KpnI and AluI restriction endonucleases. Top Keywords 16SrXII-A phytoplasma subgroup, Echinacea purpurea, nested PCR, RFLP. Top |