Phytopathogenic Mollicutes
Year : 2011, Volume : 1, Issue : 2
First page : ( 87) Last page : ( 90)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/j.2249-4669.1.2.015

Screening for phytoplasma presence in West Herzegovina vineyards

Delić Duška^1,*, Lolić Biljana¹, Karacić Ana²

¹University of Banjaluka, Faculty of Agriculture, Banjaluka-78000, Bosnia and Herzegovina

²Federal Agromediterranean Institute of Mostar, Mostar-88000, Bosnia and Herzegovina

*Corresponding author e-mail: duskadelic@yahoo.com

Online published on 21 February, 2012.

Received:  27  October,  2011; Accepted:  30  November,  2011.

Abstract

Surveys for grapevine yellows phytoplasmas in Bosnia and Herzegovina have been conducted since 2004 and vineyards in Srpska region where mostly monitored. In 2011 an extended survey was done in vineyards of West Herzegovina, the most important region for grapevine industry. Four areas were inspected for presence of phytoplasma symptoms and 57 grapevine and weed samples were collected for laboratory analyses. Nested-PCR/RFLP analyses allow to verify presence of ‘bois noir’ phytoplasma, tuf type-b in 15 symptomatic grapevine samples collected in all surveyed areas.

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Keywords

Grapevine, phytoplasmas, survey, PCR/RFLP analyses, ‘bois noir’.

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