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Phytopathogenic Mollicutes
Year : 2015, Volume : 5, Issue : 1-Suppl.
First page : ( S25) Last page : ( S26)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2015.00010.9

Phytoplasmas associated with naturally infected Catharanthus roseus in La Paz, Baja California Sur, Mexico

Poghosyan Arevik*, Hernandez-Gonzalez Julio, Lebsky Vladimir

Centro de Investigaciones Biologicas del Noroeste (CIBNOR), La Paz, BCS, Mexico

*Corresponding author e-mail: Arevik Poghosyan (arevik04@cibnor.mx)

Online published on 2 January, 2015.

Abstract

Phytoplasma infection was detected in periwinkle (Catharanthus roseus) with multiple yellows-type disease symptoms including virescence, phyllody, virescence of petals and proliferation of shoots in La Paz, Baja California Sur, Mexico. The phytoplasma presence was first studied by scanning electron microscopy. Using nested PCR, cloning, sequencing and virtual RFLP analysis phytoplasmas belonging to two ribosomal subgroups have been identified and classified.

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Keywords

Phytoplasma, periwinkle, SEM, nested PCR, virtual RFLP.

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