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Molecular diversity of phytoplasmas infecting cherry trees in Poland Ciesliñska Miroslawa*, Smolarek Terezia Institute of Horticulture, Plant Protection Department, Pomologiczna 18, 96–100, Skierniewice, Poland *Corresponding author e-mail: Miroslawa Cieœliñska (Miroslawa.Cieslinska@inhort.pl)
Online published on 2 January, 2015. Abstract Sour cherry with shoot proliferation and dieback symptoms and sweet cherry showing leaf roll and yellowing of the leaves were observed in orchards located in west and central parts of Poland. Results of phylogenetic and RFLP analysis of the partial sequence of 16S rDNA and 16S-23S rDNA spacer region virtually digested with 16 restriction enzymes showed the presence of ‘Candidatus Phytoplasma prunorum’ in sweet cherry and ‘Ca. P. asteris’ in sour cherry trees. Top Keywords Cherry, phytoplasma, detection, PCR-RFLP, sequencing, phylogeny. Top | |
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