First report of mixed infection of phytoplasmas and begomoviruses in eggplant in India Singh Jitender1,*, Singh Aastha1, Kumar Pankaj1, Rani Anchal1, Baranwal Virendra K.2, Sirohi Anil1 1College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India 2Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India *Corresponding author e-mail: Jitender Singh (jeets-80@rediffmail.com)
Online published on 2 January, 2015. Abstract Symptoms associated with yellows disease of eggplants were observed in fields of Meerut district of India. Total DNA extracted from symptomatic and asymptomatic plants was subjected to nested-PCR assay using phytoplasma 16S rDNA primers and generic PCR with universal begomovirus coat-protein (CP) gene primers. Amplicons of expected size were obtained from symptomatic plants in both PCR assays. No amplifications were observed from asymptomatic plants. The eggplant phytoplasma was identified as a member of group 16SrVI, while the begomovirus showed 99% of CP gene identity with other whitefly transmitted begomoviruses. Top Keywords Eggplant, brinjal, phytoplasmas, begomoviruses, nested-PCR. Top |