Evaluation of the synergistic effect of Curcuma aromatica in combination with sorafenib on a hepatoblastoma cell line In vitro Huynh Thanh-Tuan1,*, Le Thi-Do Quyen2,**, Vo Thanh-Hoa3,***, Le Kieu-Minh4,****, Hoang Thanh-Chi5,*****, Bui Thi-Kim Ly6,****** 1Center for Training and Scientific Research, Tam Anh General Hospital, Ho Chi Minh City, Vietnam 2Cho Ray Hospital, Ho Chi Minh City, Vietnam 3College of Pharmacy, Taipei Medical University, Taipei, Taiwan 4Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam 5Department of Medicine and Pharmacy, Thu Dau Mot University, Binh Duong Province 6Department of Medicine and Pharmacy, Thu Dau Mot University, Binh Duong Province *Corresponding Author E-mail: tuanht@hcm.tahospital.vn
**drquyen2003@gmail.com
***339107005@tmu.edu.tw
****lkminh@ump.edu.vn
*****chiht@tdmu.edu.vn
******lybtk@tdmu.edu.vn
Online Published on 10 October, 2023. Abstract Liver cancer is one of the most common cancers, accounting for the sixth-highest number of new cases and the third-highest number of deaths. Sorafenib is a well-known targeted therapy for the treatment of liver cancer. Sorafenib's therapeutic use has also been linked to side effects such as diarrhea, hypertension, and skin toxicity. As a result, combining Sorafenib with other therapeutic agents is required to avoid unwanted side effects. In this study, we tried to test the combination effect of Sorafenib and Curcuma aromatica extract. The cytotoxic, migration, and colony assays were used to investigate the conjugated effect of C. aromatica extract and Sorafenib on liver cancer cell growth inhibition. At the same Sorafenib concentration, the more C. aromatica extract supplemented, the higher the cell death rate, with a statistically significant difference. To assess the relative potency of the combination, an isobologram model integrated into the CalcuSyn software was used. All of the data points were located in a synergistic area below the additive line, according to the schematic isobologram and CI distribution chart. The results indicated that the effect of sorafenib and C. aromatica was synergistic. C. aromatica extract could be used to help treat liver cancer when used with sorafenib. Top Keywords Curcuma aromatica, Sorafenib, Hep J5, Cytotoxicity, Liver cancer. Top |