Combinational antidiabetic effects of Curcuma longa L. and Trigonella foenum-graecum L. extracts in alloxan induced type-1 diabetic rats Shaik Sadak Basha1, Shaik Sameena Fathima2, Shaik Mannur Ismail3,*, Sarbon Norizah Mhd3, Meesala Guru Sekhar4, Aluru Sudheer5, Pasupuleti Sreenivasa Rao6, Arifullah Mohammed7, Rahman Suzanah Abdul8, Nannepaga John Sushma9, Mohiddin Syed Siraj10, Ramesh Bellamkonda11, Dornadula Giri Raja Sekhar12, Matcha Bhaskar10 1Department Humanities and Sciences, Annamacharya Institute of Technology and Sciences, Rajampet, Andhra Pradesh, India 2Department of Biochemistry, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India 3Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia 4Department of Zoology, Government Degree College, Cumbum, Prakasam, Andhra Pradesh, India 5Department of Molecular Genetics, Thalassemia and Sickle Cell Society, Hyderabad, Telangana State, India 6Department of Biochemistry, Narayana Medical College and Hospital, Nellore-524003, Andhra Pradesh, India 7Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, 17600, Jeli, Kelantan, Malaysia 8Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia 9Department of Biotechnology, Sri Padmavathi Mahila Visvavidyalam, Tirupati-517502, India 10Department of Zoology, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India 11Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, USA 12Department Pharmacy, Annamacharya Institute of Technology and Sciences, Rajampet, Andhra Pradesh, India *Corresponding Author E-mail: mannurismail@umt.edu.my
Online Published on 5 February, 2024. Abstract Diabetes mellitus is a metabolic disorder that is one of the leading causes of death globally. The current study aimed to determine the combined protective effect of Curcuma longa (CL) and Trigonella foenum-graecum (TFG) on alloxan-induced oxidative stress in liver tissue of albino Wister rats. Rats were divided into 8 groups with 6 individuals each. Group I served as control, group II, III and IV were treated with CL, TFG and CL+TFG respectively. Group V, VI, VII and VIII were alloxan-induced diabetic rats, whereas group V served as negative control and group VI, VII and VIII were treated with CL, TFG and CL+TFG respectively. Blood glucose, body weight, oxidative enzymes and histopathological changes were evaluated. Group V showed significant increase in blood glucose levels after induction of alloxan, whereas, group VI, VII and VIII showed significantly decreased levels. However, the best hypoglycaemic activity was found in group VIII. The body weights were slightly increased in groups II, III and IV after the treatment of plant extracts, whereas, decreased body weights were noticed in group V. though no significant weight changes were observed in groups VI, VII and VIII. A significant decrease in oxidative enzyme levels as compared to group V were recorded. The histopathological investigation of liver tissue showed morphological alteration in group V and ameliorative effects in group VI, VII and VIII due to administration of CL, TFG and CL+TFG. Therefore, the combined administration of CL+ TFG exhibits the highest hypoglycemic activity by reducing the oxidative enzyme levels and regeneration of liver tissue. Top Keywords Curcuma longa, Trigonella foenum-graecum, Antidiabetic activity, Oxidative enzymes, Liver histology. Top |