Phytochemical Screening and Green Synthesis of Antibacterial Silver Nanoparticles of Sapindus mukorossi Fruit Extracts Ale Yogita1,*, Rana Shilpa1, Nainwal Nidhi1, Rawat Shivani2, Butola Mansi1, Zainul Rahadian3, Jakhmola Vikash1 1Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun-248007, India 2Department of Pharmaceutical Science and Technology, Sardar Bhagwan Singh University, Dehradun-248161, India 3Department of Chemistry, Faculty of Mathemetics and Natural Sciences, Universitas Negeri Padang, Indonesia *Corresponding Author E-mail: yogitaale7@gmail.com
Online Published on 07 February, 2024. Abstract Background Plants, herbs, and ethnobotanicals have been utilized for health promotion and disease treatment since the dawn of time and are still widely employed today. The immense demand and potential health advantages of herbal medicines balance out the enormous research demands. There is a need for research on the quality, safety, molecular effects, and therapeutic efficacy of the many common herbs. Aim and Objective The aim of the study is to prepare Silver Nanoparticles using fruit extract of Sapindus Mukorossi by green synthesis technique, and to develop new derivatives of antibiotic due to high resistance from conventional antibiotics. Method The Sapindus mukorossi fruit extract AgNPs was prepared by green synthesis technique. Formulation of silver nanoparticles using water/methanol reetha fruit extract was carried out by addition of fruit extract to a reaction mixture containing 1M AgNO3 and 0.625% ammonia solution and left for 48hrs in dark place with regular interval exposing to light. The prepared silver nanoparticles were characterized using SEM, FTIR and XRD studies. Result The synthesized nanoparticles found to be spherical and crystalline in nature. The FTIR measurements identifies the functional groups attributed for silver ions reduction and AgNPs stabilization. The synthesized yield was 58.34%, 77.46%, 49.67% and 69.32% respectively. The methanol extract shows greater activity than Aqueous extract against E. coli and Streptococcus aureus. Conclusion The Phyto-based drugs exhibit good medicinal properties with less side-effects as compare to synthetic based drugs. The reetha is known for its many properties such as foaming agent, antibacterial activity etc. The idea was to develop the new antibacterial drugs as the humans getting resistant to the antibacterial drugs. The antibacterial results show that the prepared AgNPs were effective in inhibiting growth of both S. aureus and E. coli bacteria, and hence they could be potential applications in bio-related field. Top Keywords Silver Nanoparticles, Sapindus mukorossi, Percentage Drug Yield, Phytochemicals, Biosynthesis. Top |