Effect of Irrigation Solution of Sodium Hypochlorite, Ethylenediamine Tetraacetic Acid, Chitosan Oligosaccharide and Agitation Techniques on Calcium Hydroxide Removal in Root Canal: In Vitro Study Heptania Melia1, Abidin Trimurni1,*, Prasetia Widi1, Gani Basri A.2,** 1Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia 2Department of Oral Biology, Faculty of Dentistry, Universitas Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia *Corresponding Author E-mail: tri.murni@usu.ac.id
**basriunoe@unsyiah.ac.id
Online Published on 07 February, 2024. Abstract Root canal treatment removes all infected pulp tissue and shapes the root canal to be appropriately filled to prevent bacteria from re-entering the root canal. Chitosan oligosaccharide is a promising irrigation solution because it has high solubility and low molecular weight, biocompatibility, good adhesion, and chelating properties.This study aims to assess the impact of various irrigation solutions, namely sodium hypochlorite, ethylenediamine tetraacetic acid, chitosan oligosaccharide, and different agitation strategies, on the efficacy of calcium hydroxide removal within the root canal. A total of 24 single-root mandibular premolars were extracted, decolonized, and root canal treated using Ca(OH)2. Examination of residual Ca(OH)2 in the channel was examined with a Scanning Electron Microscope (SEM). SEM results are assessed based on scoring with a double-blind method. Chitosan oligosaccharide irrigant with ultrasonic agitation can remove residual (Ca(OH)2) on the surface of the root canal wall, which is very good compared to the aspirator with XP Endo Finisher. The 2.5%NaOCl + 17% EDTA solution with ultrasonic agitation was better than the agitation with XP Endo Finisher. The ultrasonic agitation technique removes residue (Ca(OH)2) better than the XP endo finisher technique after being influenced by 2.5% NaOCl irrigation solution, 17% EDTA, and 2% chitosan oligosaccharides.The ultrasonic agitation technique generally removed up to 70% residue compared to the XP endo finisher agitation technique (30%) from all cervical, middle, and apical parts of the root canal: The ultrasonic agitation technique removes residue (Ca(OH)2 more than the XP Endo Finisher technique. Chitosan Oligosaccharide 2% has a better effect on removing residue (Ca(OH)2 in root canals. In comparison, 2% NaOCl irrigation solution is better than 17% EDTA in removing debris (Ca(OH)2). Top Keywords Agitation, Calcium hydroxide, Chitosan oligosaccharide, Ultrasonic, XP Endo Finisher. Top |