Journal of Veterinary Parasitology
Year : 2006, Volume : 20, Issue : 1
First page : ( 77) Last page : ( 79)
Print ISSN : 0971-6157.

Immunodiagnosis of fasciolosis by Dot-ELISA

Saxena M.K., Ram Hira, Banerjee P.S., Yadav C.L., Garg Rajat

Department of Parasitology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, India

Abstract

Fasciolosis is one of the major parasitic diseases causing heavy economic losses to livestock production. The conventional methods of diagnosis such as detection of eggs in faeces by sedimentation and flotation techniques have limitations while modern serology based tests like enzyme-linked immunosorbent assay (ELISA) requires sophisticated laboratory facilities. In the present study, a rapid and simple test (Dot-ELISA) was developed for diagnosis of bubaline fasciolosis using low molecular weight fractions of the parasite. The test was compared with egg detection in bile sediment and indirect plate ELISA. Dot-ELISA was found to be 100% sensitive and 52.5% specific, whereas plate ELISA was 90.74% sensitive and 42.5% specific when compared to bile egg sedimentation technique. The findings indicate that Dot-ELISA using low molecular weight protein fractions of F. gigantica can detect the disease and can be used under field conditions.

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Keywords

Dot-ELISA, Fasciola gigantica, Low molecular weight proteins, Buffalo.

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